EVALUATION OF RAPID UREASE TEST IN DIAGNOSIS OF H.PYLORI AMONG GASTRODUDENITIS AND PEPTIC ULCER BIOPSY IN COMPARISON WITH PCR IN KHARTOUM STATE

  • Waad Abdelmonem Elsheikh Ahmed 1. Department of medical microbiology, faculty of medical laboratory sciences, Alneelain University.

Abstract

Background: Recently Helicobacter pylori (H. pylori) has been involved in many serious diseases such as peptic ulcer and ended with gastric cancer, which can be prevented using specific diagnostic test and treatment.

Objective: The aim of this study was to evaluate rapid urease test (RUT) using  Polymerase Chain Reaction (PCR) as gold standard method, for the detection of the H. pylori in gastric and duodenal  biopsy specimens of gastrodudenitis and peptic ulcer , in Khartoum state, Sudan.

Materials and Methods: Fifty patients with gastrodudenitis and peptic ulcer attending Alribat Teaching hospital during period from March to April were enrolled in this study. Two gastric/duodenal biopsies specimens were collected and subjected for RUT and PCR for detection of 16-S gene. Sensitivity, specificity of RUT was calculated using PCR as gold standard.

Results out of 50 specimens tested 26 were positive (52%) and 24 were negative (48%) by using PCR. Out of 26 positive by PCR 22 were positive by RUT and out 24 negative by PCR 21 were negative by RUT .The sensitivity, specificity of RUT were 84.6% and 87.5%, respectively.

Conclusion in Comparison with PCR , RUT show high specificity and sensitivity. So its use will serve as good alternative to PCR particularly in resources limited area.

Key words: Helicobacter pylori, PCR, rapid urease test, Sudan.

Published
2017-10-30
How to Cite
Ahmed, W. A. E. (2017). EVALUATION OF RAPID UREASE TEST IN DIAGNOSIS OF H.PYLORI AMONG GASTRODUDENITIS AND PEPTIC ULCER BIOPSY IN COMPARISON WITH PCR IN KHARTOUM STATE. Journal of Biomedical and Pharmaceutical Research, 4(3). Retrieved from http://www.jbpr.in/index.php/jbpr/article/view/196
Section
Research Articles