CHROMATOGRAPHIC FINGERPRINT ANALYSIS OF FLAVONOIDS OF ACHYRANTHES ASPERA LINN. BY HPTLC

Abstract

The study was carried out with an aim to determine the chemical profile and flavanoid composition of the medicinally important plant Achyranthes aspera Linn. from methanolic extract of root, stem and leaf. Preliminary phytochemical screening was done followed by HPTLC studies. Ethyl acetate: Formic acid: Glacial acetic acid : water 10:0.5:0.5:1.3 was used as mobile phase for the separation of flavanoids. The flavanoid fingerprint of leaf exhibited 9 peaks each in methanolic neutral, acidic and basic fractions, while that of root exhibited 8 in neutral, 11 in acidic and 7 in basic methanolic extracts. The fingerprint of stem revealed the presence of 8 peaks in neutral, 9 in acidic and 7 in basic methanolic fractions. The study revealed diverse forms of flavonoids in large number in the root, stem and leaf of Achyranthes aspera Linn. It can be concluded that HPTLC fingerprint analysis of root, stem and leaf extract of Achyranthes aspera Linn. can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker.

Key words: Achyranthes aspera Linn. Root, stem, Leaf, Phytochemical Screening, HPTLC Fingerprinting, flavonoids.