MICROBIAL SENSITIVITY ASSAY & HPTLC ANALYSIS OF TERMINALIA CATAPPA & ADATHODA VASICA

  • Geetha Unnikrishnan1*, Shweta V. Bhangale2, Anju Unnithan3, Sonali Patil3 1* Department of Zoology, Birla College of Arts, Science & Commerce, Kalyan- 421304, India 2 Department of Bioanalytical sciences, Birla College of Arts, Science & Commerce, Kalyan- 421304, India 3 Department of Biotechnology, Birla College of Arts, Science & Commerce, Kalyan- 421304, India

Abstract

In the present study anti- bacterial acivity of Terminalia catappa & Adathoda vasica was evaluated to find the zone of inhibition and to set a HPTLC profile of these plant extracts to make out these plants as a source to formulate novel naturally derived antimicrobial formulations. Agar well diffusion assay of hydro- ethanol extract showed a dose dependent growth inhibitory effect on test organisms with maximum activity in Terminalia catappa extracts with an MIC in the range of 0.1 - 3 mg /ml. Whereas Adhathoda vasica showed moderate activity against test organisms. Phytochemical analysis explored the presence of different phytochemicals in the extract with maximum amount of saponins. The HPTLC fingerprinting studies showed significant fingerprint profile patterns in mobile phase Toluene: methanol (8:1, v/v/v) with maximum bands. The results suggest that Terminalia catappa leaf extracts could serve as a source of compounds to formulate herbal medicines against bacterial infections.

Published
2014-08-30
How to Cite
Sonali Patil3G. U. S. V. B. A. U. (2014). MICROBIAL SENSITIVITY ASSAY & HPTLC ANALYSIS OF TERMINALIA CATAPPA & ADATHODA VASICA. Journal of Biomedical and Pharmaceutical Research, 3(4). Retrieved from http://www.jbpr.in/index.php/jbpr/article/view/291
Issue
Vol. 3 No. 4 (2014)
Section
Research Articles

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