STAINING OF HISTOLOGICAL SECTIONS FROM THE SMALL INTESTINE USING HIBISCUS SABDARIFFA

  • Abd-Alhafeez Ibnouf1*, Khalid Adam2, Esam AbdulRaheem3, Ali Ageep4 1 Department of Medical Laboratories, Portsudan Ahlia College, Portsudan, Sudan. 2 Department of Molecular Biology, Faculty of Laboratory Medical Science, Neelain University, Khartoum, Sudan. 3 Department of Medical Laboratories, College of Applied Medical Science, Shaqra University, Saudi Arabia. 4 Department of Pathology, Red Sea University, Portsudan, Sudan.

Abstract

Objective: this was a descriptive study aimed to assess the quality of staining of intestinal tissue by Hibiscus Sabdariffa solution compared to Hematoxylin-Eosin routine stain.

Methods: paraffin-embedded formalin-fixed tissue sections from small intestine were stained by Hibiscus Sabdariffa solution using different concentrations and time durations in room temperature.

Results: With 5 % solution, 48 % of slides showed at least very good quality of staining. The excellent results were mainly obtained when time duration was 60 minutes while poorest results were obtained when staining time was 1-2 minutes

Conclusion: Hibiscus Sabdariffa solution is a cheap, natural, and harmless dye that can be used for staining of histological sections and it is comparable to the routine Hematoxylin and Eosin stain.

Key words: Hibiscus Sabdariffa; Histological Staining; Small Intestine

Published
2014-10-30
How to Cite
Ali Ageep4A.-A. I. K. A. E. A. (2014). STAINING OF HISTOLOGICAL SECTIONS FROM THE SMALL INTESTINE USING HIBISCUS SABDARIFFA. Journal of Biomedical and Pharmaceutical Research, 3(5). Retrieved from http://www.jbpr.in/index.php/jbpr/article/view/298
Issue
Vol. 3 No. 5 (2014)
Section
Research Articles

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