THE ASSOCIATION OF ki67 PROLIFERATION MARKER WITH ESTROGEN, PROGESTERONE AND HER2 MARKERS AMONG SUDANESE FEMALES WITH BREAST CANCER

Reem Mohammed Fadl Elmoula*1 Hassan Elsiddig Hassan Farag Alla**2

Reem Mohammed Fadl Elmoula*1 Hassan Elsiddig Hassan Farag Alla**2 *1 M.s.c Department of Histopathology and Cytology Sudan University of Science and Technology, Sudan. **2 Assistant Professor of Histopathology and Cytology (SUST).

Abstract

Background: Breast cancer aggressiveness can be correlated with proliferation status of tumor cells, which can be ascertained with tumor grade and Ki67 indexing.

We therefore aimed to determine associations of the Ki67 index with other prognostic markers like tumor size, grade, ER, PR and HER2 status.This study aimed to determine the association of ki67 tumor marker with ER, PR, and HER2 markers among Sudanese females with breast cancers during January to august 2012.

Methods: This is a descriptive retrospective study, aimed to detection the association of ki67 tumor marker with PR, ER, HER2 as well as clinicopathological data of breast cancer among Sudanese female attending Radiation and isotope center-Khartoum. 50 Paraffin blocks that were previously diagnosed as breast carcinoma were randomly selected and each paraffin block two sections of 3µm thick one for H&E stain and anther to immunohistochemicaln stain was performed by the DAKO envision method.

Results: out of 50 Paraffin blocks that were previously diagnosed as breast carcinoma, 47(94%) show invasive ductal carcinoma, the majority of the samples 31 (62%) were found to be grade III, the age of the involved patients range between 20 to 80 years old; the majority of the patients (more than 80%) were between the age group 41 to 50 years old, Twenty nine out of 50 sample (58%) were positive for ki67and the remaining 21(42%) were not expressed the marker.

Conclusion: the study present the ductal carcinoma is the most common 47(94%) among 50 Sudanese patients suffering from breast cancer, most of the cases diagnosed with grade III.

Keywords: breast cancer,ki67, progesterone receptor, estrogen receptor, HER2-neu, Sudanese females, haematoxylin and eosin, Immunohistochemical staining.