PRELIMINARY SCREENING OF SECONDARY METABOLITES AND BRINE SHRIMP LETHALITY BIOASSAY OF WARM-WATER EXTRACT OF PUFFER FISH ORGANS TISSUES, TETRAODON CUTCUTIA, AVAILABLE IN BANGLADESH.

Abstract

This study is aimed to establish the invitro screening of secondary metabolites namely alkaloids, steroids, alcoholic compounds, glycosides, and amide group containing compounds from warm-water extracts of puffer fish dorsal flesh, head, tail and liver tissues by conventional laboratory procedures. In secondary metabolites screening, the warm-water extract of puffer fish liver tissues excepting fish dorsal, head, and tail extract showed positive test of alkaloids, gycosides and alcoholic group test while gave negative test of steroid, amide group containing compounds. This property is found to similar to TTX, which was isolated from puffer fish available in Japan. On the other hand, fish extracts from dorsal, head, and tail showed the positive test against glycosides, alcohol, and amide but not for steroids, and alkaloids. To observe bioactivity, the warm-water crude extracts were prepared at the concentration of 02, 04, 08, 16, 32, and 64 µg/ml, and their bioactivity were tested using Brine shrimp lethality bioassay against larva Artemia Leach. The LC₅₀ (µg/ml) of the different extracts were calculated by prohibit analysis. The fish tissues extract exhibited toxicity against larva Artemia Leach, and LC₅₀ were 0.75, 0.90, 1.30, and 1.45 µg/ml but other extracts of puffer fish did not show toxicity. Further characterization is still needed for the identification of compound found in puffer fish liver extract, and would be considered as the potential candidates for new drug research.

KEY WORDS: Secondary metabolites, Tetraodon cutcutia, and bioactivity