MOLECULAR CHARACTERIZATION OF THE 16S RRNA GENE OF HELICOBACTER PYLORI ISOLATED FROM STOMACH BIOPSY IN GASTRITIS PATIENTS

  • Hiba Nugdalla Allah1, Abdorahman 2, *Walid A. H. Eldaif 2 1 B.Sc. Medical Microbiology, Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Al Neelain University, Sudan. 2 B.Sc. M.Sc. Ph.D. Medical Microbiology, Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Al Neelain University, Sudan.

Abstract

Background: Helicobacter pylorus is now accepted as the most important cause of gastritis in human. There is a growing body of literature on the detection of H. pylori 16-s gene in grastritis patients. The study was aimed to determine the frequency of H .pylori (16-S) gene, in patients with gastritis at Khartoum State, Sudan.

 

Methods: Molecular testing for H. pylori 16-S gene was done on 40 stomach biopsy using PCR technique. Biopsy specimens was collected by gastroenterologist using endoscopy .one biopsy of inflamed stomach was collected in sterile normal saline  and kept at -20OC  till used. Extraction was done by using ml jena bioscience kit. The amplification reaction was carried out in thermo cycler machine PCR system with program system consisting of an initial denaturation step at 940 c for 10 minute and 30 cycle of denaturation at 940c for 1 minute

 

Results: Twenty one samples (52.5%) out of 40 were positive by PCR, while 19 samples (47.5) were negative.

 

Conclusion: The frequency of H. pylori among endoscopic patients was 52.5%, so the frequency of infection was high.

Published
2013-12-30
How to Cite
*Walid A. H. Eldaif 2H. N. A. A. 2. (2013). MOLECULAR CHARACTERIZATION OF THE 16S RRNA GENE OF HELICOBACTER PYLORI ISOLATED FROM STOMACH BIOPSY IN GASTRITIS PATIENTS. Journal of Biomedical and Pharmaceutical Research, 2(6). Retrieved from http://www.jbpr.in/index.php/jbpr/article/view/357
Issue
Vol. 2 No. 6 (2013)
Section
Research Articles

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