IN VITRO EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF AN INDIAN MEDICINAL PLANT: CELASTRUS PANICULATUS WILLD

  • Pradeep K Naik Centre of Excellence in Natural Products and Therapeutics Department of Biotechnology and Bioinformatics, Sambalpur University, Jyotivihar, Burla, Sambalpur-768019, Odisha, India
  • Debasmita Dubey Centre of Excellence in Natural Products and Therapeutics Department of Biotechnology and Bioinformatics, Sambalpur University, Jyotivihar, Burla, Sambalpur-768019, Odisha, India
  • Priya Cheruvanachari Centre of Excellence in Natural Products and Therapeutics Department of Biotechnology and Bioinformatics, Sambalpur University, Jyotivihar, Burla, Sambalpur-768019, Odisha, India
  • Animesh Pattnaik Centre of Excellence in Natural Products and Therapeutics Department of Biotechnology and Bioinformatics, Sambalpur University, Jyotivihar, Burla, Sambalpur-768019, Odisha, India
  • Swaraj K Babu Centre of Excellence in Natural Products and Therapeutics Department of Biotechnology and Bioinformatics, Sambalpur University, Jyotivihar, Burla, Sambalpur-768019, Odisha, India
Keywords: Antibiotic resistance, Medicinal plants, Celastrus Paniculatus, Antibacterial activity, Phytochemical Analysis, Antioxidant activity

Abstract

Aim: To evaluate the antibacterial efficacy, the presence of different phytoconstituents of the leaf extracts of Indian plant Celastrus paniculatus against 6 antibiotic-resistant bacteria along with the evaluation of their antioxidant potentiality.

Methods: The leaf extracts were prepared using 4 solvents, water, methanol, n-butanol, and acetone. The antibacterial property was evaluated using agar well diffusion and micro-broth dilution method. Qualitative phytochemical analysis was performed using standard protocols for each solvent extract to check for the presence of the secondary metabolites and phytochemicals which attributes to the antibacterial effectivity of C. paniculatus. Further, the antioxidant potentiality of all the 4 leaf-solvent-extracts was estimated using the DPPH method.

Results: The n-butanol leaf extract exhibited the highest antibacterial and antioxidant activity, followed by acetone, methanol extracts. The aqueous extracts exhibited negligible antibacterial activity.

Conclusion: This study validated the antibacterial properties of C. paniculatus leaf extracts against both gram-positive and negative bacteria and the phytochemical analysis revealed the presence of many secondary importance which can be attributed to its therapeutic properties. Hence, this plant can be further used as a complementary or alternative choice of drug for combatting multidrug-resistant bacterial pathogens.

Keywords: Antibiotic resistance; Medicinal plants; Celastrus Paniculatus; Antibacterial activity, Phytochemical Analysis; Antioxidant activity

Published
2020-03-12
How to Cite
Pradeep K Naik, Debasmita Dubey, Priya Cheruvanachari, Animesh Pattnaik, & Swaraj K Babu. (2020). IN VITRO EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF AN INDIAN MEDICINAL PLANT: CELASTRUS PANICULATUS WILLD. Journal of Biomedical and Pharmaceutical Research, 9(1). https://doi.org/10.32553/jbpr.v9i1.726
Section
Research Articles