Md. Nazrul Islam4, Sreska Shrestha1*, Sanjay Yadav2*, Fuleshwar Mandal2, Mrigendra Amatya3, 1Department of Medical Laboratory Technology, Chitwan Medical College, Chitwan, Nepal 2Department of Biochemistry, Chitwan Medical College, Chitwan, Nepal. 3Department of Physiology, Nepal Medical College, Kathmandu, Nepal. 4Department of Physiology, Chitwan Medical College, Chitwan, Nepal., Nepal